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بحث بيانات أعضاء هيئة التدريس - جامعة الزقازيق
بحث بيانات أعضاء هيئة التدريس
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الموقع الشخصى
تيسير رفعت ابراهيم السيد
الكلية الطب - القسم الباثولوجيا (علم الامراض)
الباثولوجيا علم الامراض
الدرجات العلمية
بكالوريوس فى الطب والجراحه, -جامعة الزقازيق [1998]
ماجستير فى الباثولوجيا علم الامراض, -جامعة الزقازيق [2005]
دكتوراه فى الباثولوجيا علم الامراض, -جامعة الزقازيق [2008]
(إظهار وفاعلية السيتوكيراتين 19 وال - بى 21 فى السرطان الحلمى للغدة الدرقية)
الحالة الوظيفية الحالية :
قائم بالعمل
التدرج الوظيفى الأكاديمى
معيد : 9/10/2000
مدرس مساعد : 23/3/2005
مدرس : 26/8/2008
أستاذ مساعد : 25/11/2014
أستاذ : 29/12/2020
الأبحاث العلمية
1 -
Immunohistochemical Study of Glypican-3 and HepPar-1 in Differentiating Hepatocellular Carcinoma from Metastatic Carcinomas in FNA of the Liver (1435).
2 -
Immunohistochemical expression of HBME-1 and galectin-3 in thedifferential diagnosis of follicular-derived thyroid nodules (1435).
3 -
Immunohistochemical Expression of Aldehyde Dehydrogenase-1 and Hypoxia- Inducible Factor-1α in Breast Cancer (1435).
4 -
Expression and Significance of Ezrin and E-cadherin in Colorectal Carcinoma (1435).
5 -
IMMUNOHISTOCHEMICAL EXPRESSION OF COX -2 AND CD44 (STEM CELL MARKER) IN URINARY BLADDER CANCER (1435).
6 -
EXPRESSION AND SIGNIFICANCE OF PTEN AND GLUT-1 IN PROLIFERATIVE ENDOMETRIUM, ENDOMETRIAL HYPERPLASIA AND ENDOMETRIOID ADENOCARCINOMA. (1435).
7 -
Influence Of Sylimarin On Valproic Acid Induced Hepatotoxicity In Adult male Albino Rats (1434).
8 -
clinical utility of caspase-cleaved cytokeratine 18 fragments in viral- related chronic liver disease patients (1431).
9 -
Diagnostic Utility of Immunohistochemical Expressions of IMP3 Versus DOG1 and p63 in Salivary Gland Tumors (1441).
10 -
Clinicopathological characterization and prognostic implication of FOXP3 and CK19 expression in papillary thyroid carcinoma and concomitant Hashimoto’s thyroiditis (1441).
11 -
Prognostic Value of TWIST1 and EZH2 Expression in Colon Cancer (1441).
12 -
Clinicopathological and Prognostic Value of Immunohistochemical Expression of CD44 (Stem Cell Marker) and Ki67 in Serous Ovarian Cancer (1441).
13 -
Expression of Stem Cell Marker OCT4 and Epidermal Growth Factor Receptor Predict Recurrence in Transitional Cell Carcinoma of Bladder in Egyptian Patients (1441).
14 -
Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients (1441).
15 -
The utility of immunohistochemical expression of glypican-3 (GPC3) and enhancer of zeste homologue-2 (EZH2) protein in the diagnosis of liver nodules (1438).
16 -
Clinicopathological significance and correlation of fatty acid synthase (FASN) and HER-2 expression in infiltrating duct carcinoma of the breast (1438).
المجالات البحثية
Clinicopathological and Prognostic Value of Immunohistochemical Expression of CD44 (Stem Cell Marker) and Ki67 in Serous Ovarian Cancer Taiseer R. Ibrahim, Samar M. Abdelraouf, Mohamed Abdelgawad, Amira Elwan Abstract Introduction: Failure of effective treatment of epithelial carcinoma of the ovary necessitates searching factors that lead to resistance to chemotherapy and recurrence. Cancer stem cell theory hypothesis provides an explanation of how small group of cells inside the tumour remain resistant to treatment. CD44 has been considered as cancer stem cell marker in multiple malignancies but its role in Ovarian Serous Carcinoma (OSC) is still controversial. Aim: To investigate the expression of stem cell marker CD44 and proliferative activity using Ki67 immunostaining in serous ovarian carcinoma and their relation to clinical and pathological variables and outcome of the patients. Materials and Methods: The present study was a prospective study done on 45 patients with serous ovarian tumours, the ovarian sections obtained from the patients were immunostained by CD44 and Ki67 immunohistochemical markers. The Overall Survival (OS), Disease Free Survival (DFS) and response to treatment were investigated and analysed. Results: Both CD44 and Ki67 were positive in patients with increased grade and advanced stage and the difference was highly statistically significant (p=0.001 and p<0.001, respectively). CD44 and Ki67 expression were higher in bilateral cancer with presence of lymph node and distant metastasis than in unilateral tumours without lymph node and distant metastasis. The present study found positive relation between CD44 and Ki67 expression (p=0.006). CD44 and Ki67 positive expression level in OSC had a significantly shorter 3-year DFS than the patient group with negative expression of CD44 and Ki67 (p=0.020 and p=0.002, respectively). Conclusion: This study demonstrated that CD44 and Ki67 expression were significantly associated with high grade and late stage serous ovarian carcinoma, and their high expression was associated with poor prognosis and resistance to chemotherapy, so proper quantification may play a vital promising function in choosing targeting therapy and predicting more better result outcome.
Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients Mai M. Abdelwahab, Huda F. Ebain , Taiseer R. Ibrahim, Mohamed S. Badr, Mohamed E. Lashin, Mahamoud Abdou Yassin, Adel M. Ismail, Ahmed A. Obaya Abstract Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of Galac-tin-1 (Gal-1) and its tissue immunohistochemical expression in SOC patients at different stages trying to find out its significance as a diagnostic and prognostic marker. Patients and methods: The study included 95 females I-Control group: Twenty five healthy females; II-Patients group: Seventy females diagnosed as SOC at different stages; Stage I: 8 cases, Stage II: 12 cases, Stage III: 32 cases and Stage VI:18 cases. Serum Galectin-1 and CA-125 were measured by ELIZA and tissue Galectin-1 was assessed by immunohistochemistry. All patients were followed for up to 3 years after surgery. Results: Serum Gal-1 and CA-125 levels were significantly high in SOC patients compared to controls (p < 0.001). We found a direct positive statistically significant correlation between serum Gal-1 and CA125 levels (p < 0.001). Se-rum Gal-1 at cut off value > 135 ng/ml was superior to CA-125 a cut off value > 49 u/ml with sensitivity, specificity of 100%, vs 88.57, 96% for CA-125. Serum Gal-1 was significantly associated with tumor stage (p < 0.001). Immunohistochemistry showed that patients with strong Gal-1 expression had higher serum level (p = 0.002). Stromal and tumor Gal-1 expression were significantly correlated with tumor grade (p < 0.001) and stage (p = 0.001). Se-rum Gal-1, CA-125 and IHC Gal-1 expression were associated with poor survival (p < 0.001, p = 0.009 and p = 0.002) respectively. Conclusion: Serum Gal-1 and its tissue IHC expression are useful diagnostic and prognostic markers for SOC patients.
Clinicopathological characterization and prognostic implication of FOXP3 and CK19 expression in papillary thyroid carcinoma and concomitant Hashimoto’s thyroiditis Salem Y. Mohamed, Taiseer R. Ibrahim, Samah S. Elbasateeny, Lobna A. Abdelaziz, Shaimaa Farouk, Mahamoud Abdou Yassin, Ahmed Embaby Abstract Background: Papillary thyroid carcinoma (PTC) is considered the most prevalent thyroid malignancy. The association between Hashimoto’s thyroiditis (Ht) and(PTC) is still unclear. Objective: We aimed to examine the clinicopathological impact of mmunohistochemical staining of FOXP3 and Cytokeratin 19 in PTC and concomitant Ht and their correlation with patients’ outcome and survival. Methods: Eighty thyroid biopsies obtained from patients with PTC were immunostained by FOXP3 and CK19.The patients were treated by radioactive iodine (I 131) and followed up. Results : FOXP3 and CK19 expression were detected in 45% and 80% studied cases of PTC respectively. 16.7% of PTC with associated HT showed FOXP3+lymphocytes in lymphocytic infiltrate of HT, while most of PTC associated HT express cytoplasmic CK19 positive Hurtle cells. FOXP3 was more expressed in PTC female patients more than 45 years with higher stage, lymph node, and distant metastasis, extracapsular extension, number of i131doses, and cumulative radioiodine doses with a highly statistically significant difference (p < 0.001). The relation was significant between CK19 immunostaining as regard 10-year Overall Survival and death (p value = 0.027 and 0.036, respectively). Conclusions: HT represents a step in the process of autoimmune inflammatory disease ending by the evolution of PTC with better prognosis; therefore appropriate follow up of these cases is needed. FOXP3 tends to be more expressed in PTC cases with worse prognostic variables and is predictable to become a recent prognostic and targeted therapy for (PTC). There was a significant relation between CK19 immunostaining and 10 year overall survival.
Clinicopathological significance and correlation of fatty acid synthase (FASN) and HER-2 expression in infiltrating duct carcinoma of the breast Samah Said Elbasateeny1, Taiseer Refat Ibrahim1, Ibtsam Shehta Harera2 ABSTRACT Objective: This study aimed to correlate the immunohistochemical expression of fatty acid synthase (FASN) and HER-2 with the clinicopathological variables in breast infiltrating duct carcinoma (IDC) to identify the impact of marker expression on tumor behavior and mammary carcinogenesis and to detect the correlations between the two markers. Methods: Immunohistochemical expressions of FASN and HER-2 were evaluated in 77 breast case including 10 normal breast, 12 ductal carcinoma in situ (DCIS) and 55 IDC and correlated with clinicopathological variables. The obtained data were statistically analyzed. Results: FASN was overexpressed in 91.7% and 70.9% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.000). In IDC, the expression of FASN was significantly more expressed in low-grade IDC (P = 0.031). FASN was significantly upregulated in larger tumors and lymph node metastasis (P = 0.017 and P = 0.046, respectively). The expression of FASN had a significant negative correlation with progesterone receptor (PR) (P = 0.05). HER-2 was overexpressed in 50% and 25.45% of DCIS and IDC, respectively, with a significant difference from the normal (P = 0.026). HER-2 was only expressed in high-grade DCIS (P = 0.007). In IDC, the expression of HER-2 was significantly up-regulated in larger tumors and lymph node metastasis (P = 0.033 and P = 0.015, respectively). The expression of HER-2 had a significant negative correlation with estrogen receptor (ER) and PR (P = 0.001 and P = 0.023, respectively). Correlation between the expression of FASN and HER-2 in IDC, revealed a significant positive correlation (Spearman correlation [r] = 0.374, P = 0.005). Conclusions: We concluded that overexpression of FASN and HER-2 may facilitate the early diagnosis of IDC. In IDC, their over expression might warn of a more aggressive course. Our correlation found that FAS expression is closely associated with over expression of HER-2 in IDC.
Diagnostic Utility of Immunohistochemical Expressions of IMP3 Versus DOG1 and p63 in Salivary Gland Tumors Taiseer R. Ibrahim, Mona M. Ahmed, Abdelmonem A.Hegazy Abstract Objective: The diverse site of origin and classification complexity of salivary glands tumors increase difficulties in their diagnosis. This study aimed to evaluate the specificity and diagnostic ability of immunohistochemical expressions of IMP3 versus DOG1 and p63 in cases of such tumors. Material and Method: Thirty paraffin-embedded salivary gland tumors were obtained from the Pathology Department Archive. Their diagnosis was confirmed. The specimens were then re-classified and evaluated using the IMP3, DOG1 and p63 immunohistochemical markers. Results: There were 8 pleomorphic adenoma (PA), 12 mucoepidermoid carcinoma (MEC) and 10 adenoid cystic carcinoma (ADC) cases. All 12 MECs (100%) were IMP3 positive, while 30% of ADCs and only 25% of PAs were positive for IMP3. There was a statistically significant relationship between salivary gland tumors and IMP3 immunostaining (P =0.03). As regards to DOG1 results, 12.5% of PAs showed variable luminal positive immunostaining and 40% of ADCs showed weak luminal and abluminal immunostaining while 16.7% of MEC showed cytoplasmic staining. On the other hand, all ADCs (100%) showed moderate p63 reactivity in the nuclei of abluminal cells. All MEC cases (100%) were also p63-positive, showing a strong diffuse nuclear reactivity. A statistically significant relationship was noticed between salivary gland tumors and p63 immunostaining (P <0.05). Conclusion: IMP3 is more sensitive for diagnosis of MEC than ADC. p63 is statistically significant in diagnosing salivary gland tumors (MEC and ADC). On the other hand, DOG1 staining is not sensitive in diagnosis of studied malignant salivary gland tumors, limiting its diagnostic utility.
Expression of Stem Cell Marker OCT4 and Epidermal Growth Factor Receptor Predict Recurrence in Transitional Cell Carcinoma of Bladder in Egyptian Patients Mona M. Ahmed ,Taiseer R. Ibrahim, Safa A.Balata, Mohamed Malek, Ibrahim Ismail, Ayman Omar Abstract Introduction: In the past, the prognosis of bladder cancer was dependent on the presence of muscle invasion. Recently High-Grade T1 (HGT1) Transitional Cell Carcinoma (TCC), found to be associated with similar behaviour and prognosis even following Bacillus Calmette Guerin (BCG) therapy. Recognition of new markers that may help in selection of high-risk cases that need aggressive treatment even in the early stage to avoid unwanted toxicity of chemotherapy has to be high priority. Aim: To evaluate the prognostic value of Octamer-Binding Transcription Factor 4 (OCT4) and Epidermal Growth Factor Receptor (EGFR) immunohistochemical expression in urothelial carcinoma of the bladder and their effect as well as the vascular invasion on the recurrence of these tumours. Materials and Methods: Prospective cohort study conducted in Zagazig University hospital from January 2010 till December 2013. Immunohistochemical staining of OCT4, EGFR and Cd34 for 68 cases of bladder cancer samples was done. The correlation between the expressions of each marker and clinic-pathologic parameters was then analyzed. Results: OCT4 was detected in 53 patients (77.9%) of total cases. EGFR showed high expression in 25 of cases. 18 and 25 cases revealed low and no EGFR expression, respectively. There was a significant association between intensity of OCT4 expression and tumour grade (p<0.001), muscle invasion (p<0.001), vascular invasion (p=0.004) and stage (p=0.001). Significant association was established between EGFR expression and tumor size, grade, lamina propria, muscle and vascular invasion and stage (p 0.005, <0.001, 0.001, <0.001, respectively). Significant association observed between OCT4, EGFR expression and vascular invasion and recurrence (p=0.026, 0.001 and 0.002 respectively). Conclusion: Using selective inhibitors for EGFR and OCT4 especially that approved for EGFR in treating selected cases of urothelial carcinoma of bladder could suppress tumor growth. These markers in addition to presence of vascular invasion have to be used in screening of high risk noninvasive group that benefits from target therapy
Prognostic Value of TWIST1 and EZH2 Expression in Colon Cancer Samar M. Abdelraouf , Taiseer R. Ibrahim, Lobna A. Abdelaziz, Mohamed I. Farid, Salem Y. Mohamed Abstract Background :Colorectal carcinoma (CRC) is the third most common human cancer. Twist, a basic helix-loop-helix (bHLH) transcription factor, is an epithelial-mesenchymal transition ((EMT) inducer that has been involved in carcinogenesis and chemoresistance. Also, the enhancer of Zeste homolologue2 (EZH2), a member of the polycomb group of genes, had been associated with poor prognosis in several malignancies. Objective :To evaluate the expression of Twist1 and EZH2 in colon carcinoma in Egyptian patients and its relation to clinico-pathological parameters, prognosis, and survival. Methods: Twist1 and EZH2 expressions were evaluated immunohistochemically in 50 cases of colorectal tumors (12 colon adenomas and 38 colon carcinomas) and 20 samples from normal colonic mucosa. Results :The expression of Twist1 and EZH2 was significantly higher in colon adenoma and carcinoma than that in normal colonic mucosa (P< 0.05). Twist1 and EZH2 expressions were associated with decreased tumor differentiation, advanced stage, and lymph node metastasis. Twist1 and EZH2 expressions were significantly related to 3-year disease-free survival (P=0.005 and 0.002 respectively) and 3-year overall survival (P= 0.045 and 0.039, respectively). Conclusions:Twist1 and EZH2 may serve as prognostic predictors for colon carcinoma and may have a potential role as therapeutic targets in patients with colon carcinoma in the future.
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